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The Potent Inhibitory Effect of β-D-Mannuronic Acid (M2000) as a Novel NSAID with Immunosuppressive Property on Anti-Cyclic Citrullinated Peptide Antibodies, Rheumatoid Factor and Anti-dsDNA Antibodies in Patients with Rheumatoid Arthritis

[ Vol. 14 , Issue. 3 ]

Author(s):

Hossein Ahmadi, Ahmad R. Jamshidi, Mahdi Mahmoudi, Salvatore Cuzzocrea, Mohammad Javad Fattahi, Anis Barati, Bernd H. A. Rehm, Hidenori Matsuo and Abbas Mirshafiey   Pages 206 - 214 ( 9 )

Abstract:


Objective: To investigate the inhibitory effect of β-D-mannuronic acid (M2000) on anti-cyclic citrullinated peptide antibodies (anti-CCP), rheumatoid factor (RF), antidouble strand DNA (anti-dsDNA) and acute phase reactants in rheumatoid arthritis (RA) patients.

Methods: The study included 40 patients with RA who had an inadequate response to conventional therapy (identifier: IRCT2014011213739N2). The patients were permitted to continue the conventional therapy excluding NSAIDs. 21 of them were treated orally by M2000 at a dose of 500 mg twice daily for 12 weeks and the others did not. Serum samples were collected at baseline, 4 weeks and 12 weeks after treatment and were tested for the serum level of anti-CCP and anti-dsDNA antibodies using enzyme linked immunosorbent assay. The serum level of RF and C-reactive proteins (CRP) was determined by the immunoturbidimetric assay, respectively.

Results: At baseline, all patients in the M2000 treated group and the control group were positive for anti-CCP, RF. moreover, 4 of 21 (19%) in the M2000 treated group and 2 of the 19 (10.5%) patients in the control group were positive for anti-dsDNA antibodies, respectively. The serum levels of anti-CCP, RF and anti-dsDNA were decreased significantly after M2000 therapy (p<0.001, p<0.001 and p<0.001, respectively). The reduction in the level of anti-CCP was positively correlated with disease activity, swollen joint count and CRP. Furthermore, the level of inflammatory markers ESR and CRP decreased significantly after M2000 therapy (p<0.001 and p<0.004, respectively).

Conclusion: M2000 shows inhibitory effect on anti-CCP, RF, anti-dsDNA antibodies and acute phase reactants in RA patients.

Keywords:

Rheumatoid arthritis, M2000, Anti-CCP, RF, Anti-dsDNA.

Affiliation:

Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Rheumatology Research Center, Shariati Hospital, Tehran University of Medical Science, Tehran, Rheumatology Research Center, Shariati Hospital, Tehran University of Medical Science, Tehran, Department of Biological and Environmental Sciences, University of Messina, Messina, Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Institute of Fundamental Sciences, Massey University, Private Bag 11222, Palmerston North 4442, Department of Neurology, National Hospital Organization Nagasaki Kawatana Medical Center, and Department of Neuroimmunology, Nagasaki University Graduate School of Biomedical Sciences, Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Box: 14155-6446, Tehran

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