Asis Bala*, Chaitali Mondal and Pallab Kanti Haldar Pages 1 - 5 ( 5 )
Zebrafish have a similar hepatic anatomy and cellular architecture with mammals. Therefore, number of investigators are using zebrafish to study liver pathologies. However, the evaluation model specific to liver toxicities in zebra fish was not clearly stated earlier. The present study was designed to develop a model of embryonic liver toxicity using dexamethasone (DEXA, 0-20 µM) as standard hepatotoxic agent and such toxicities easily measured by streptavidin conjugated peroxidase assay after 48 hour post fertilization (hpf) of DEXA treatment. In addition to morphological toxicities at different hpf, DEXA showed significant (*p< 0.05 & **p<0.01) reduction of peroxidase- chromogenic dye reaction in the assay as compared to DEXA untreated embryos at 10 & 20 µM concentration that concluded the hepatocellular toxicity of dexamethasone. Hopefully the developed model for hepatotoxicity evaluation will be a promising model for evaluation of investigational new drugs or chemicals as an easy vertebrate model before commencement of another animal model.
Zebra fish, embryonic liver toxicity, Streptavidin conjugated peroxidase assay
Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Hajipur, EPIP, Industrial Area, Vaishali 844102, Bihar, TCG Life Sciences (Chembiotek) Pvt. Ltd., Sector V, Salt Lake Electronics Complex, Kolkata, West Bengal 700091, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, West Bengal