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Evaluation of Diuretic and Antioxidant Properties in Aqueous Bark and Fruit Extracts of Pine


Hadi Shariati, Mohammad Hassanpour, Gholamreza Sharifzadeh, Asghar Zarban*, Saeed Samarghandian and Farhad Saeedi   Pages 1 - 7 ( 7 )


Objective: The present study has been carried out to evaluate the diuretic and antioxidant properties of pine herb in an animal model.

Materials and Methods: 45 adult male rats were randomly divided into nine groups including: groups I (the negative control), groups II (positive control, furosemide 10 mg/kg), groups III to VIII (treatment groups received 100, 200, 400 mg/kg of the aqueous extracts of bark and fruit) and group IX received the combination of aqueous extract of bark (100 mg/kg) and the fruit (100 mg/kg). The urine output, glomerular filtration rate (GFR), electrolytes, urea, and creatinine levels were evaluated . Furthermore, the phenolic content and antioxidant activity of both extracts were also assessed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and Folin–Ciocalteu methods.

Results: The aqueous extracts of the pine bark and fruit increased the urinary output in a dose-dependent manner. The combination of the two extracts compared to the other extracts alone significantly increased the serum potassium level. This study also showed each extract increase creatinine clearance in a dose-dependent manner (p<0.01 and p<0.05). The increase of GFR in the combination group was not significant. The current data showed a significant increase in the total phenolic content in pine bark extract in compared with the fruit extract.

Conclusion: The pine bark and fruit can be useful in the prevention and treatment of kidney stones due to the high antioxidant activity.


Antioxidant, Diuretics, Kidney stones, Phenolic content, Pine


General surgery department, Faculty of medicine, Golestan University of Medical Sciences, Gorgan, Pharmacology Department, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Social Determinants of Health Research Center, Birjand University of Medical Sciences, Birjand, General surgery department, Faculty of medicine, Golestan University of Medical Sciences, Gorgan, Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Student Research Committee, Birjand University of Medical Sciences, Birjand

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