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Extraction, Chemical Composition, Antioxidant Property, and In-vitro Anticancer Activity of Silymarin from Silybum marianum on Kb and A549 Cell Lines

Author(s):

Mojgan Azadpour, Mohammad Mehdi Farajollahi, Ali Mohammad Varzi, Pejman Hashemzadeh, Hossein Mahmoudvand and Mitra Barati*   Pages 1 - 7 ( 7 )

Abstract:


Introduction: This study aimed to evaluate the antioxidant property of silymarin (SM) extracted from the seed of Silybum marianum and its anticancer activity on KB and A549 cell lines following 24, 48, and 72 h of treatment.

Methods: Ten grams of powdered S. marianum seeds were defatted using n-hexane for 6 hours and then extracted by methanol. The silymarin extracted of extraction components The extracted components of silymarin were measured by spectrophotometric assay and HPLC analysis. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, phenol content, total flavonoid content, and total antioxidant capacity were measured to detect the antioxidant properties of SM. The anticancer activity of the SM on cell lines evaluated by MTT.

Results: In HPLC analysis, more than 50% of the peaks were related to silibin A and B. SM was reducedDPPH (the stable free radical) with a 50% inhibitory concentration (IC50) of 6.56 μg/ ml in comparison with butylated hydroxyl toluene (BHT), which indicated an IC50 of ~3.9 μg/ ml.The cytotoxicity effect of SM on the cell lines was studied by MTT assay. The cytotoxicity effect of the extracted silymarin on KB and A549 cell lines was observed up to 80 and 70% at 156 and 78 µg/ml, respectively. The IC50 value of the extracted SM on KB and A549 cell lines after 24 hours of treatment was seen at 555 and 511 µg/ml, respectively.

Conclusion: Due to the good antioxidant and anticancer properties of the isolated silymarin, its use as an anticancer drug is suggested.

Keywords:

Cytotoxicity, MTT Assay, KB Cell line, A549 Cell line.

Affiliation:

Research Center of Pediatric Infectious Disaeses, Hazrat-e-Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Faculty of Science, Department of Medical Biotechnology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Facutuly of Science, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Facutuly of Science, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Facutuly of Science, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Research Center of Pediatric Infectious Disaeses, Hazrat-e-Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran



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