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Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry for Drug Development

[ Vol. 7 , Issue. 3 ]

Author(s):

Andrea L.O.Hebb, Virender Bhan, Alexander D. Wishart, Craig S. Moore, George S. Robertson and Yunsheng Hsieh   Pages 223 - 231 ( 9 )

Abstract:


Hydrophilic interaction chromatography (HILIC) offers a difference in selectivity as compared to traditional normal phase and reversed-phase liquid chromatography and therefore has great potential for the separation of a variety of pharmaceuticals. This review is devoted to summarizing HILIC coupled to tandem mass spectrometric (MS/MS) methods for the rapid, sensitive and accurate determinations of small molecules employed for supporting drug discovery. The perspectives and challenges on performing HILIC-MS/MS assays are also presented.

Keywords:

Hydrophilic interaction chromatography, tandem mass spectrometry, pharmaceutical analysis, drug development, HILIC, mass spectrometric (MS/MS) methods, HPLC, electrospray ionization, ESI, atmospheric pressure, APCI, atmospheric pressure pho-toionization, APPI, Re-versed-phase liquid chromatography, Ultrahigh-pressure liquid chromatography, liquid, –, liquid extraction, trifluoroacetic acid, solid-phase extraction, SPE, pro-tein precipitation, PPT, ZWITTERIONIC HILIC-MS/MS, ZIC, AMINO-MODE HILIC-MS/MS, CAPILLARY HILIC-MS/MS, GABA, ULTRA-PERFORMANCE HILIC-MS/MS, Caco-2 assay, phosphatidylcholine, lysophosphatidylcholine

Affiliation:

Department of Pharmacology, Dalhousie University, Sir Charles Tupper Medical Building, Faculty of Medicine, 5850 College Street, Halifax, Nova Scotia, B3H 1X5, Canada., Department of Drug Metabolism and Pharmacokinetics, Merck Research Laboratories, 2015 Galloping Hill Road, K-15-3700, Kenilworth, NJ 07033, USA.



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