Asis Bala*, Chaitali Mondal and Pallab KantiHaldar Pages 735 - 739 ( 5 )
Background: Zebrafish have similar hepatic anatomy and cellular architecture just like mammals. Therefore, a number of investigators are using zebrafish to study liver pathologies. However, the evaluation model specific to liver toxicities in zebra fish was not clearly stated earlier. Aims and Objectives: The present study was designed to develop a model of embryonic liver toxicity using dexamethasone (DEXA, 0-20 μM) as a standard hepatotoxic agent.
Methods: such toxicities are easily measured by streptavidin-conjugated peroxidase assay after 48- hour post-fertilization (hpf) of DEXA treatment.
Results: In addition to morphological toxicities at different hpf, DEXA showed significant (*p< 0.05 &**p<0.01) reduction of peroxidase-chromogenic dye reaction in the assay as compared to DEXA untreated embryos at 10 & 20 μM concentration that concluded the hepatocellular toxicity of dexamethasone.
Conclusion: Hopefully, the developed model for hepatotoxicity evaluation will be a promising model for the evaluation of new drugs or chemicals as an easy vertebrate model before the commencement of another animal model.
Zebra fish, embryonic liver toxicity, streptavidin conjugated peroxidase assay, toxicology, hepatic anatomy, mammals.
Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Hajipur, EPIP, Industrial Area, Vaishali 844102, Bihar, TCG Life Sciences (Chembiotek) Pvt. Ltd., Sector V, SaltLake Electronics Complex, Kolkata, West Bengal 700091, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, West Bengal